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Alternatives to Laboratory Animals - ATLA

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Phenotypic anchoring of arsenic and cadmium toxicity in three hepatic-related cell systems reveals compound- and cell-specific selective up-regulation of stress protein expression: Implications for fingerprint profiling of cytotoxicity.

Gottschalg, E., Moore, N.E., Ryan, A.K., Travis, L.C., Waller, R.C., Pratt, S., Atmaca, M., Kind, C.N. and Fry, J.R.

Chemico-Biological Interactions, 161, 251-261 (2006).

Exposure of cells to toxic chemicals is known to up-regulate the expression of a number of stress proteins (SPs), including metallothionein (MT) and members of the heat shock protein (HSP) family, and this response may allow the development of a fingerprint profile to identify mechanisms of toxicity in an in vitro toxicology setting. To test this hypothesis, three hepatic-derived cell culture systems (rat hepatoma FGC4 cell line, rat hepatocytes, human hepatoma HepG2 cell line) were exposed to cadmium (as CdCl2) and arsenic (as NaAsO2), two compounds believed to exert their toxicity through an oxidative stress mechanism, under conditions of phenotypic anchoring defined as minimal and mild toxicity (approximately 5 and 25% reduction in neutral red uptake, respectively). The expression of six SPs – MT, HSP25/27, HSP40, HSP60, HSP70, and HSP90 – was then determined by ELISA. Expression of four of these SPs – MT, HSP25/27, HSP40 and HSP70 – was up-regulated in at least one experimental condition. However, the patterns of expression of these four SPs varied across the experimental con