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ATLA - ISI
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Alternatives to Laboratory Animals - ATLA

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Short note: evaluation of a commercial kit for glutathione estimation in cellular systems.


Atmaca, M., Hammond, A.H. and Fry, J.R.

ATLA, 25(6), 667-673 (1997).

The major aim of this study was to identify an appropriate assay to use routinely for cellular glutathione measurement. The Saville assay has been widely used in cytotoxicity studies, while the GSH-400 assay is a commercial kit which only recently became available. Therefore, in this study, the accuracy and sensitivity of the Saville and GSH-400 assays were compared. Results presented herein indicate that the Saville assay gave a lower blank absorbance and higher sensitivity when compared to the GSH-400 assay.