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Use of the SOS-chromotest Spot Assay as a Screening System for Detecting Genotoxic Compounds in Crude Plant Extracts

Daniel Arrieta Baez, Gerardo Zepeda Vallejo, Patricia Cano Sánchez, Matilde Breña Valle, Ricardo Reyes Chilpa and Manuel Jiménez Estrada

An SOS-chromotest spot assay was used to detect genotoxic compounds in crude plant extracts. The method allows simultaneous testing of extracts from different species in either a liquid or a solid crystalline form. Extracts from two species of the genus Senna, native to the state of Morelos, Mexico, were assayed. Four genotoxic compounds were isolated, and were identified as quercetin and rutin from S. wislizeni, and 5,7-di-O-methylrutin and 5,7-di-O-methylquercetin from S. skinneri. The SOS-chromotest spot assay proved to be useful for activity-guided fractionation at the beginning of screening for genotoxic compounds in crude plant extracts.