The highly controversial Median Lethal Dose Test, or LD50, was developed by J. W. Trevan in 1927 as a means to determine characteristics in acute toxicity. However, due to the necessity to terminate large numbers of test subjects as a requisite of the test it received heavy criticism. In 1991 the UK Government decided upon its full ban in research, as the benefits of the test ultimately did not outweigh the overall harm being cause.

For more information visit the European Commission’s webpage.